Journal: Advanced Science
Article Title: Investigating the Interactions of Glioma Stem Cells in the Perivascular Niche at Single‐Cell Resolution using a Microfluidic Tumor Microenvironment Model
doi: 10.1002/advs.202201436
Figure Lengend Snippet: GB3‐RFP cells maintain stem phenotype within 3D microfluidic devices. a) GB3‐RFP cells demonstrated positive staining for (i) Nestin, (ii) CD44 (iii) SOX2 markers. b) GB3‐RFP cells demonstrated positive staining for proliferative markers (i) EdU when co‐stained with Nestin and (ii) negative for GFAP when co‐stained with Ki‐67. Furthermore (iii) GB3‐RFP cells co‐stained with Nestin and GFAP demonstrated positive staining for Nestin but were negative for GFAP. Scale bar: 20 µm. (Monoculture; Mono, Coculture with astrocytes; Co‐A, Co‐culture with vasculature; Co‐V)
Article Snippet: The antibodies used were anti‐Human Nestin (1:200, (10C2) Mouse NB300‐266 (Novus Biologicals)), anti‐GFAP (1:400, (GA5) Mouse mAb #3670 (Cell Signaling Technologies)), anti‐SOX2 (1:400, (D6D9) XP Rabbit mAb #3579, (Cell Signaling Technologies)), anti‐Ki‐67 (1:1000, Rabbit ab15580 (Abcam)), mouse CD31 (10 μg mL ‐1 , (P2B1) (Developmental Studies Hybridoma Bank)).
Techniques: Staining, Co-Culture Assay